The rank of major may refer to:
- Zero, real name David Oh, the former commander of the FOX Unit, who held the rank of major during his command of the unit up to its disbandment.
- Ivan Raidenovitch Raikov, a GRU officer who held the rank of major durings the events of Operation Snake Eater and the San Hieronymo Incident.
- Revolver Ocelot, a Russian Spetsnaz soldier who held the rank of major during the 1960s while leading the Ocelot Unit.
- Richard Ames, a DIA agent (and secretly an agent of the Patriots), who held the rank of major until his promotion to colonel after the events of the Shadow Moses Incident for his role in the event.
- The Major, the commander of Rogue Coyote, who held the rank of major while serving in the Army.
- Vince, the chief of SaintLogic's security forces in Metal Gear Acid 2, who formerly held the rank of Major.
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