Playable characters[]
- Big Boss
- Solid Snake
- Venom Snake
- Raiden
- Gray Fox (VR Missions; Null; and DLC costume)
- Big Boss's resistance
- Jonathan
- FOX Soldier
- USSR Male & Female Soldier
- Male & Female Officer
- Male & Female Scientist
- Government official
- Soviet Engineer
- Nikolai Stepanovich Sokolov
- Ivan Raidenovitch Raikov
- EVA (non-canon)
- Sigint (non-canon)
- Para-Medic (non-canon)
- Gene (non-canon)
- Cunningham (non-canon)
- Elisa and Ursula (non-canon)
- Python
- Ocelot (non-canon)
- Venus (non-canon)
- Teliko Friedman (non-canon)
- GA-KO soldier
- U.S. local soldiers (US only; password)
- Japanese local soldiers (JPN only; password)
- KGB soldier (EU only)
- Special Camo Soldier (JPN only; password)
- Militaires Sans Frontières soldiers
- Diamond Dogs soldiers
- Samuel Rodrigues (DLC)
- Blade Wolf (DLC)
- Custom Avatar/Venom Snake
- Captain
Playable characters (online playing)[]
- FOX Soldier
- USSR Male & Female Soldier
- Male & Female Officer
- Male & Female Scientist
- Soviet Engineer
- Government official
- KGB soldier
- GRU soldier
- Ocelot Unit
- Ivan Raidenovitch Raikov
- Nikolai Stepanovich Sokolov
- European local soldiers (EU only)
- Venom Snake
- Quiet
- Revolver Ocelot
- Custom Avatar